Paul Dyce, Ph.D.

 

Assistant Professor, Reproductive Developmental Biology

Department of Animal Sciences

295 CASIC Building

559 Duvall Drive

Auburn University, AL, 36849

(334) 844-1840

Education

M.Sc. Animal and Poultry Science, University of Guelph, Canada

Ph.D. Animal and Poultry Science, University of Guelph, Canada

Experience

Assistant Professor 2015-present

Department of Animal Sciences

Auburn University, Auburn, AL, USA

 

Adjunct Professor 2018-present

Department of Animal Nutrition and Feed Science

Sichuan Agricultural University, Sichuan, China

 

Adjunct Professor 2015-2018

Department of Animal Biosciences, OAC

University of Guelph, Ontario, Canada

 

Research Associate 2014-2015

Department of Animal Biosciences, OAC

University of Guelph, Ontario, Canada

 

CIHR Postdoctoral Research Fellow 2010-2014

Department of Physiology and Pharmacology

Schulich School of Medicine and Dentistry

Western University, London, Ontario, Canada

 

ANSC-3600 Reproductive Physiology

ANSC-4980 Undergraduate Research

My research focuses on several key areas:

1) Studying the ability of fetal porcine and postnatal mouse skin isolated stem cells to form germ cells under various in vitro culture conditions. The ability of stem cells to form germ cells in vitro opens up many possibilities to study germ cell formation under controlled in vitro conditions.

2) I am also interested in the exploration of intra-ovarian signaling pathways required for normal oocyte/embryo development within livestock species. This has the potential to improve in vitro oocyte techniques such as growth, maturation, and cryopreservation within agriculturally relevant species.

3) Develop molecular methods to improve heifer and gilt selection for reproductive potential in order to improve the productivity and efficiency of these valuable agriculture sectors.

Original Research Articles 

  1. Yan CH, Li L, Liu JC, Wang YF, Liu XF, Ge W, Dyce PW, Li, L, Sun XF, Shen W, Cheng SF (2019) RA promotes proliferation of primordial germ cell-like cells differentiated from porcine skin-derived stem cells. J Cell Physiol. DOI: 10.1002/jcp.28454. [Epub ahead of print]
  2. Dickinson SE, Elmore MF, Kriese-Anderson L, Elmore JB, Walker BN, Dyce PW, Rodning SP, Biase FH (2019) Evaluation of age, weaning weight, body condition score, and reproductive tract score in preselected beef heifers relative to reproductive potential. Journal of Animal Science and Biotechnology. 10:18 [Epub ahead of print]
  3. Ge W, Li L, Dyce PW, De Felici M, Shen W (2019) Establishment and depletion of the ovarian reserve: physiology and impact of environmental chemicals. Cell Mol Life Sci. 2019Feb 27. doi: 10.1007/s00018-019-03028-1. [Epub ahead of print]
  4. Zhang TY, Sun XF, Li L, Ma JM, Zhang RQ, Li N, Liu XL, Dyce PW, Shen W (2019) Ochratoxin A exposure impairs porcine granulosa cell growth via the P13K-AKT signaling pathway. Journal of Agriculture and Food Chemistry. 2019Feb 25. doi: 10.1021/acs.jafc.8b06361. [Epub ahead of print]
  5. Levesque CL. Akhtar N, Huynh E, Walk C, Wilcock P, Zhang Z, Dyce PW, de Lange CFM, Khafipour E, Li J (2018) The impact of epidermal growth factor supernatant on pig performance and ileal microbiota. Translational Animal Science. 2(2):184-194. doi:10.1093/tas/txy019.
  6. Zhai QY, Ge W, Wang JJ, Sun XF, Cheng SF, Liu JC, Li L, Zhao Y, Dyce PW, De Felici M, Shen W (2018) Exposure to zinc oxide nanoparticles during pregnancy induces oocyte DNA damage and affects ovarian reserve of mouse offspring. Aging. 10(8):2170-89. doi: 10.18632/aging.101539.
  7. Phillips KM, Read CC, Kriese-Anderson LA, Rodning SP, Brandebourg TD, Biase FH, Marks ML, Elmore JB, Standford MK, Dyce PW (2018) Plasma metabolomic profiles differ at the time of artificial insemination based on pregnancy outcome, in Bos taurus beef heifers. Scientific Reports. 8(1):13196. doi: 10.1038/s41598-018-31605-0.
  8. Dye ZT, Dyce PW (2018) Editorial on “PNLDC1 is essential for piRNA 3’ end trimming and transposon silencing during spermatogenesis in mice”. Translational Cancer Res. 7(Suppl 7):S755-S757. doi: 10.21037/tcr.2018.07.26.
  9. Read CC, Willhelm G, Dyce PW (2018) Connexin 43 coupling in bovine cumulus cells, during the follicular growth phase, and its relationship to in vitro embryo outcomes. Molecular Reproduction and Development. Accepted for publication. doi: 10.1002/mrd.22993.
  10. Wei Y, Liu Z, Xu K, Huyhn E, Dyce PW, Li J, Zhou W, Dong S, Feng B, Mu Y, Li J, Li K (2018) Generation and propagation of cluster of differentiation 163 biallelic gene editing pigs. Scientia Agricultura Sinica. 2018;51(4): 770-777. doi: 11-1328/S
  11. Dyce PW, Tenn N, Kidder GM (2018) Retinoic acid enhances germ cell differentiation of mouse skin-derived stem cells. Journal of Ovarian Research. 11(1):19. doi: 10.1186/s13048-018-0390-3.
  12. Medeiros S, Xie J, Dyce PW, Cai H, DeLange K, Zhang H, Li J (2018) Isolation of bacteria from fermented food and grass carp intestine and their efficiencies in improving nutrient value of soybean meal in solid state fermentation. Journal of Animal Science and Biotechnology. 9:29. doi: 10.1186/s40104-018-0245-1.
  13. Dickinson SE, Griffin BA, Elmore MF, Kriese-Anderson L, Elmore JB, Dyce PW, Rodning SP, Biase FH (2018) Transcriptome profiles in peripheral white blood cells at the time of artificial insemination discriminate beef heifers with different fertility potential. BMC Gen. 19(1):129. doi: 10.1186/s12864-018-4505-4.
  14. Wang JJ, Ge W, Liu JC, Klinger FG, Dyce PW, De Felici M, Shen W (2017) Complete in vitro oogenesis: retrospects and prospects. Cell Death Differ. 24(11):1845-1852. doi: 10.1038/cdd.2017.134.
  15. Sun YC, Ge W, Lai FN, Zhang RQ, Wang JJ, Cheng SF, Shen W, Dyce PW (2017) Oocyte-like cells induced from CD34-positive mouse hair follicle stem cells in vitro. J Genet Genomics. 44(8):405-407. doi: 10.1016/j.jgg.2017.08.001.
  16. Liu JC, Lai FN, Li L, Sun XF, Cheng SF, Ge W, Wang YF, Li L, Zhang XF, De Felici M, Dyce PW, Shen W (2017) Di (2-ethylhexyl) phthalate exposure impairs meiotic progression and DNA damage repair in fetal mouse oocytes in vitro. Cell Death Dis. 8(8):e2966. doi: 10.1038/cddis.2017.350.
  17. Sun YC, Wang YY, Ge W, Cheng SF, Dyce PW, Shen W (2017) Epigenetic regulation during the differentiation of stem cells to germ cells. Oncotarget. 8(34):57836-57844. doi: 10.18632/oncotarget.18444.
  18. Sun YC, Sun XF, Dyce PW, Shen W, Chen H (2017) The role of germ cell loss during primordial follicle assembly: a review of current advances. Int J Biol Sci. Mar 11;13(4):449-457. doi: 10.7150/ijbs.18836.
  19. Ge W, Zhao Y, Lai FN, Liu JC, Sun YC, Wang JJ, Cheng SF, Zhang XF, Sun LL, Dyce PW, Shen W (2017) Cutaneous applied nano-ZnO reduce the ability of hair follicle stem cells to differentiate. Nanotoxicology. May;11(4):465-474. doi: 10.1080/17435390.2017.1310947.
  20. Fu XF, Yang F, Cheng SF, Feng YN, Li L, Dyce PW, Shen W, Sun XF (2017) The epigenetic modifications and the anterior to posterior characterization of meiotic entry during mouse oogenesis. Histochem Cell Biol. 148(1):61-72. doi: 10.1007/s00418-017-1545-9.
  21. Fang-Nong Lai, Jing-Cai Liu, Lan Li, Jun-Yu Ma, Xue-Lian Liu, Yu-Ping Liu, Xi-Feng Zhang, Hong Chen, Massimo De Felici, Paul W. Dyce, Wei Shen (2017) Di (2-ethylhexyl) phthalate impairs steroidogenesis in ovarian follicular cells of prepuberal mice. Arch Toxicol. 2017 Mar;91(3):1279-1292. doi: 10.1007/s00204-016-1790-z.
  22. Ge W, Cheng SF, Dyce PW, De Felici M, Shen W (2016) Skin-derived stem cells as a source of primordial germ cell-and oocyte-like cells. Cell Death Dis. Nov 10;7(11):e2471. doi: 10.1038/cddis.2016.366.
  23. Li L, Liu JC, Lai FN, Liu HQ, Zhang XF, Dyce PW, Shen W, Chen H (2016) Di (2-ethylhexyl) phthalate exposure impairs growth of antral follicle in mice. PLoS One. Feb 4;11(2):e0148350. doi: 10.1371/journal.pone.0148350.
  24. Sun R, Sun Yuan-Chau, Ge Wei, Tan Hui, Cheng Shun-Feng, Yin Shen, Sun Xiao-Feng, Li Lan, Dyce P, Li Julang, Shi Qing-Hua, Shen Wei (2015) The crucial role of Activin A on the formation of primordial germ cell-like cells from skin-derived stem cells in vitro. Cell Cycle. 14(19):3016-29. doi: 10.1080/15384101.2015.1078031.
  25. Wang J, Liu Y, Sun YC, Ge W, Wang Y, Dyce P, Hou R, Shen W (2015) Basic fibroblast growth factor stimulates the proliferation of bone marrow mesenchymal stem cells in Giant Panda. PLoS One. 10(9):e0137712. doi: 10.1371/journal.pone.0137712.
  26. Ge W, Ma HG, Cheng SF, Sun YC, Sun LL, Sun XF, Li L, Dyce P, Li J, Shi QH, Shen W (2015) Differentiation of early germ cells from human skin stem cells without exogenous gene integration. Sci Rep. Sep 8;5:13822. doi: 10.1038/srep13822.
  27. Dyce PW, Li D, Barr K, Kidder GM (2014) Connexin43 is required for the maintenance of multipotency in skin-derived stem cells. Stem Cells Dev. 23(14):1636-46. doi: 10.1089/scd.2013.0459
  28. Dyce PW (2013) Differentiation of Newborn Mouse Skin Derived Stem Cells into Germ-like Cells In Vitro. J. Vis. Exp. (77):e50486. doi:10.3791/50486.
  29. Dyce PW, Norris RP, Lampe PD, Kidder GM (2012) Phosphorylation of serine residues in the C-terminal cytoplasmic tail of connexin43 regulates proliferation of ovarian granulosa cells. J. Membrane Biology, Jun; 245(5-6):291-301. doi: 10.1007/s00232-012-9460-6.
  30. Dyce PW, Liu J, Tayade C, Kidder GM, Betts DH, Li J (2011) In vitro and in vivo germ line potential of stem cells derived from newborn mouse skin. PLoS One. 6(5):e20339. doi: 10.1371/journal.pone.0020339.
  31. Dyce PW, Shen W, Huynh E, Shao H, Villagómez DA, Kidder GM, King WA, Li J (2011) Analysis of oocyte-like cells differentiated from porcine fetal skin-derived stem cells. Stem Cells Dev. May;20(5):809-19. doi: 10.1089/scd.2010.0395.
  32. Dyce PW, Toms D, Li J (2010) Stem cells and germ cells: microRNA and gene expression signatures. Histology and Histopathology 25(4):505-513. doi: 10.14670/HH-25.505.
  33. Lermen D, Gorjup E, Dyce PW, von Briesen H, Muller P (2010) Neuro-muscular differentiation of adult porcine skin derived stem cell-like cells. PLoS ONE. Jan 29;5(1):e8968. doi: 10.1371/journal.pone.0008968.
  34. Linher K, Dyce PW, Li J (2009) Primordial Germ Cell-like Cells differentiated in vitro from skin-derived stem cells. PLoS ONE. Dec; 4(12):e8263. doi: 10.1371/journal.pone.0008263.
  35. Hao Y, Wax D, Zhong Z, Murphy C, Ross JW, Rieke A, Samuel M, Spate L, Dyce PW, Li J, Sutovsky P, Prather RS (2009) Porcine skin-derived stem cells can serve as donor cells for nuclear transfer. Cloning and Stem Cells. Mar;11(1):101-10. doi: 10.1089/clo.2008.0063.
  36. Lermen D, Blomeke B, Browne R, Clark A, Dyce PW, Fixemer T, Fuhr GR, Holt WV, Jewgenow K, Lloyd RE, Lotters S, Paulus M, Reid GM, Rapoport DH, Rawson D, Ringleb J, Ryder OA, Sporl G, Schmitt T, Veith M, Muller P (2009) Cryobanking of viable biomaterials: implementation of new strategies for conservation purposes. Mol Ecol. Mar;18(6):1030-3. doi: 10.1111/j.1365-294X.2008.04062.x.
  37. Cheung QC, Yuan Z, Dyce PW, Wu D, Delange K, Li J (2009) Generation of epidermal growth factor-expressing Lactococcus lactis and its enhancement on intestinal development and growth of early-weaned mice. Am J Clin Nutr. Mar;89(3):871-9. doi: 10.3945/ajcn.2008.27073.
  38. Wen L, Craig J, Dyce PW, Li J (2006) Cloning of porcine signal transducer and activator of transcription 3 cDNA and its expression in reproductive tissues. Reproduction. Sep; 132(3):511-8. doi: 10.1530/rep.1.01055.
  39. Dyce PW, Wen L, Li J (2006) In vitro germline potential of stem cells derived from fetal porcine skin. Nature Cell Biology. Apr;8(4):384-90. doi: 10.1038/ncb1388.
  40. Dyce PW, Li J (2006) From skin cells to ovarian follicles? Cell Cycle. Jul;5(13):1371-5.
  41. Craig J, Zhu H, Dyce PW, Wen L, Li J (2005) Leptin enhances porcine preimplantation embryo development in vitro. Mol. Cell Endocrinol. Jan. 229(1-2): 141-7. doi: 10.4161/cc.5.13.2898.
  42. Craig J, Zhu H, Dyce PW, Petrik J, Li J (2004) Leptin enhances oocyte nuclear and cytoplasmic maturation via mitogen-activated protein kinase pathway. Endocrinology. Nov. 145(11): 5355-63. doi: 10.1210/en.2004-0783.
  43. Zhu H, Craig JA, Dyce PW, Sunnen N, Li J (2004) Embryos derived from porcine skin-derived stem cells exhibit enhanced pre-implantation development. Biol. Reprod. Dec. 71(6): 1890-7. doi: 10.1095/biolreprod.104.032227.
  44. Dyce PW, Zhu H, Craig J, Li J (2004) Stem cells with multilineage potential derived from porcine skin. Biophys. Res. Commun. 316:651-658. doi: 10.1016/j.bbrc.2004.02.093.
  45. Dyce PW, DeVries R, Walton J, Hacker R, Li J (2003) Inducible expression of green fluorescent protein in porcine airway epithelial cells by bovine tracheal antimicrobial peptide promoter. Biotechnol. Bioeng. Nov 5;84(3):374-81. doi: 10.1002/bit.10779.

 

Book Chapters

  1. Read CC, Phillips KM, Linher K, Li, J, Dyce PW (2019) From Stem Cell to Gamete. Comprehensive Biotechnology 3rd edition. Elsevier.
  2. Toms D, Dyce PW, Li J (2016) Differentiation potential of stem cells into ovarian cells. Frontiers in Stem Cell and Regenerative Medicine Research Published by Bentham eBooks, 2015, Vol. 2, 3-54.
  3. Dyce PW, Linher K, Li J. (2009) Somatic stem cells of non-gonadal tissues: their germline potential. Stem Cells in Reproductive Medicine, 2nd Edition, Edited by Carlos Simon and Antonio Pellicer, published by Informa Healthcare pp 69-81.

 

Undergraduate Publications

  1. Gorman SA, Read CC, Phillips KM, Brandebourg TD, Dyce PW (2019) The potential role of inflammation in oocyte health and developmental potential. Auburn University Journal of Undergraduate Scholarship.
  2. Gohlke M, Dyce P, Brandebourg T (2019) Characterization of mitochondria variation between oocytes harvested from lean and obese pigs. Auburn University Journal of Undergraduate Scholarship.

 

Research

My research focuses on several key areas:

1) Studying the ability of fetal porcine and postnatal mouse skin isolated stem cells to form germ cells under various in vitro culture conditions. The ability of stem cells to form germ cells in vitro opens up many possibilities to study germ cell formation under controlled in vitro conditions.

2) I am also interested in the exploration of intra-ovarian signaling pathways required for normal oocyte/embryo development within livestock species. This has the potential to improve in vitro oocyte techniques such as growth, maturation, and cryopreservation within agriculturally relevant species.

3) Develop molecular methods to improve heifer and gilt selection for reproductive potential in order to improve the productivity and efficiency of these valuable agriculture sectors.